Bone Morphogenetic Protein-7, Human Recombinant

pDESCRIPTION: Recombinant Human BMP-7 produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 139 amino acids and having a molecular mass of 15679.97 Dalton.br /
The rHuBMP-7 is purified by proprietary chromatographic techniques.br /
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Thr-Gly-Ser-Lys.br /
SOURCE: E.Coli Expression Systembr /
PURITY: Greater than 95.0% as determined by:br /
(a) Analysis by RP-HPLC.br /
(b) Anion-exchange FPLC.br /
(c) Analysis by reducing and non-reducing SDS-PAGE Silver-Stained gel.br /
ENDOTOXIN: Less than 0.1 ng/mu;g (IEU/mu;g) of BMP-7.br /
DIMERS amp; AGREGATES: Less than 1% as determined by silver-stained SDS-PAGE gel analysisbr /
FORM: Purified, in 10mM sodium citrate pH=3.5.br /
PROTEIN CONTENT: Protein quantitation was carried out by two independent methods:br /
1. UV spectroscopy at 280 nm.br /
2. Analysis by RP-HPLC, using a standard solution of BMP-7 as a Reference Standard.br /
CONCENTRATION: 0.1 mg/mlbr /
BIOLOGICAL ACTIVITY: rHuBMP-7 is fully biologically active when compared to standard.br /
The ED50 as determined by the cytolysis of MC3T3-E1 cells is lt; 50 ng/ml.br /
STORAGE: -20deg;C (aliquot), avoid repeated freeze and thaw cycles/p