BNP, Human
- 貨號 Cat. No.
- 11-BNP
- 規格 Size
- 5 μg
- 品牌 Brand
- ARP
Description
pDESCRIPTION: Recombinant Human BNPï€ produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 32 amino acids and having a molecular mass of 3464 kDa.br /
rHuBNP is purified by proprietary chromatographic techniques.br /
The sequence of the amino acids was determined and was found to be: SPKMVQGSGCFGRKMDRISSSSGLGCKVLRRHbr /
SOURCE: E.Coli Expression Systembr /
PURITY: Greater than 95.0% as determined by:br /
(a) Analysis by RP-HPLC.br /
(b) Anion-exchange FPLC.br /
(c) Analysis by reducing and non-reducing SDS-PAGE Silver-Stained gel.br /
ENDOTOXIN: Less than 0.1 ng/mu;g (IEU/mu;g) of rHu Beta-NGF.br /
DIMERS amp; AGREGATES: Less than 1% as determined by silver-stained SDS-PAGE gel analysisbr /
FORM: Purified, in 20mM Phosphate buffer and 0.6mM Sodium Chloride.br /
PROTEIN CONTENT: Protein quantitation was carried out by two independent methods:br /
1. UV spectroscopy at 280 nm.br /
2. Analysis by RP-HPLC, using a calibrated solution BNP ï€ as a Reference Standard.br /
CONCENTRATION: 0.5 mg/mlbr /
BIOLOGICAL ACTIVITY: rHuBNP ï€ is fully biologically active when compared to standards.br /
STORAGE: -20deg;C (aliquot), avoid repeated freeze and thaw cycles/p
rHuBNP is purified by proprietary chromatographic techniques.br /
The sequence of the amino acids was determined and was found to be: SPKMVQGSGCFGRKMDRISSSSGLGCKVLRRHbr /
SOURCE: E.Coli Expression Systembr /
PURITY: Greater than 95.0% as determined by:br /
(a) Analysis by RP-HPLC.br /
(b) Anion-exchange FPLC.br /
(c) Analysis by reducing and non-reducing SDS-PAGE Silver-Stained gel.br /
ENDOTOXIN: Less than 0.1 ng/mu;g (IEU/mu;g) of rHu Beta-NGF.br /
DIMERS amp; AGREGATES: Less than 1% as determined by silver-stained SDS-PAGE gel analysisbr /
FORM: Purified, in 20mM Phosphate buffer and 0.6mM Sodium Chloride.br /
PROTEIN CONTENT: Protein quantitation was carried out by two independent methods:br /
1. UV spectroscopy at 280 nm.br /
2. Analysis by RP-HPLC, using a calibrated solution BNP ï€ as a Reference Standard.br /
CONCENTRATION: 0.5 mg/mlbr /
BIOLOGICAL ACTIVITY: rHuBNP ï€ is fully biologically active when compared to standards.br /
STORAGE: -20deg;C (aliquot), avoid repeated freeze and thaw cycles/p
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