Artemin, human

pDESCRIPTION: Recombinant Human Artemin (rHuArtemin) produced in E.Coli is a disulfide-linked homodimer, non-glycosylated polypeptide chain containing two identical 113 amino acids and having a total molecular mass of 24213.4 Daltonbr /
The sequence of the first five N-terminal amino acids was determined and was found to be ALa-Gly-Pro-Gly-Glybr /
SOURCE: E.Coli Expression Systembr /
PURITY: Greater than 98.0% as determined by:br /
(a) Analysis by RP-HPLC.br /
(b) Anion-exchange FPLC.br /
(c) Analysis by reducing and non-reducing SDS-PAGE Silver- Stained gelbr /
ENDOTOXIN: Less than 0.1 ng/mu;g (IEU/mu;g) of rHuArteminbr /
DIMERS amp; AGREGATES: Less than 1% as determined by silver-stained SDS-PAGE gel analysisbr /
FORM: Purified, in 10mM Sodium Citrate pH 4.5, 25mM NaCl.br /
PROTEIN CONTENT: Protein quantitation was carried out by two independent methods:br /
-UV spectroscopy at 280 nm using the absorbency valuebr /
- Analysis by RP-HPLC, using a standard solution of Artemin as abr /
Reference Standardbr /
CONCENTRATION: mg/mlbr /
BIOLOGICAL ACTIVITY: rHuArtemin is fully biologically active when compared to standard.br /
The ED50, calculated by the dose-dependant proliferation assaybr /
SH-SY5Y cell line which was found to be 4-8ng/ml.br /
The activity can also determined by it's ability to promote survivalbr /
and neurite outgrowthbr /
STORAGE: -20deg;C (aliquot), avoid repeated freeze and thaw cycles/p