Angiopoietin-2, human
- 貨號 Cat. No.
- 11-ANGPO-2H
- 規格 Size
- 5 μg
- 品牌 Brand
- ARP
Description
pDESCRIPTION: Human Angiopoietin-2 (Ang-2), a 66 kDa protein consisting of 476 amino acid residuesbr /
(N21-F496), is fused to a N-terminal myc-tag and produced in insect cells.br /
The angiopoietin (Ang) family of growth factors includes four members, all of which bind tobr /
the endothelial receptor tyrosine kinase Tie2. Two of the Angs, Ang-2 and Ang-4, activate thebr /
Tie2 receptor, whereas Ang-2 and Ang-3 inhibit Ang-2-induced Tie2 phosphorylation.br /
Angiopoietin-2 (Ang-2) is a secreted growth factor which binds to and activates the Tie-2br /
receptor tyrosine kinase. The factor enhances endothelial cell survival and capillarybr /
morphogenesis, and also limits capillary permeability. Ang-2 binds the same receptor but failsbr /
to activate it: hence, it is a natural inhibitor of Ang-2. Ang-2 destabilises capillary integrity,br /
facilitating sprouting when ambient vascular endothelial growth factor (VEGF) levels are high,br /
but causing vessel regression when VEGF levels are low. Tie-1 is a Tie-2 homologue but itsbr /
ligands are unknown. Angiopoietin and Tie genes are expressed in the mammalianbr /
metanephros, the precursor of the adult kidney, where they may play a role in endothelialbr /
precursor growth. Tie-1-expressing cells can be detected in the metanephros when it firstbr /
forms and, based on transplantation experiments, these precursors contribute to the generationbr /
of glomerular capillaries. During glomerular maturation, podocyte-derived Ang-2 andbr /
mesangial-cell-derived Ang-2 may affect growth of nascent capillaries.br /
After birth, vasa rectae acquire their mature configuration and Ang-2 expressed by descendingbr /
limbs of loops of Henle would be well placed to affect the growth of this medullarybr /
microcirculation. Finally, preliminary data implicate angiopoietins in deregulated vesselbr /
growth in Wilms' kidney tumours and in vascular remodelling after nephrotoxicity. Altogether,br /
existing data suggest that VEGF-A and Angiopoietins not only have quite differentbr /
but also very complementary and coordinated roles.br /
SOURCE: Insect cellsbr /
PURITY: Greater than 95.0% as determined by:br /
(a) Analysis by RP-HPLC.br /
(b) Anion-exchange FPLCbr /
DIMERS amp; AGGREGATES: lt;1% as determined by silver stained SDS-PAGE gel analysisbr /
ENDOTOXIN: Less than 0.1 ng/mu;g (IEU/mu;g) of rhANG-2br /
PROTEIN CONTENT: Protein quantitation was carried out by two independent methods:br /
1) UV spectroscopy at 280 nm.br /
2) Analysis by RP-HPLC, using a standard solution of ANG1 as a Reference Standard.br /
FORM: Purifiedbr /
STORAGE: -20deg;C, avoid freezing and thawing cycles after reconstitutionbr /
BIOLOGICAL ACTIVITY: rhANG-2 is fully biologically active when compared to standard./p
(N21-F496), is fused to a N-terminal myc-tag and produced in insect cells.br /
The angiopoietin (Ang) family of growth factors includes four members, all of which bind tobr /
the endothelial receptor tyrosine kinase Tie2. Two of the Angs, Ang-2 and Ang-4, activate thebr /
Tie2 receptor, whereas Ang-2 and Ang-3 inhibit Ang-2-induced Tie2 phosphorylation.br /
Angiopoietin-2 (Ang-2) is a secreted growth factor which binds to and activates the Tie-2br /
receptor tyrosine kinase. The factor enhances endothelial cell survival and capillarybr /
morphogenesis, and also limits capillary permeability. Ang-2 binds the same receptor but failsbr /
to activate it: hence, it is a natural inhibitor of Ang-2. Ang-2 destabilises capillary integrity,br /
facilitating sprouting when ambient vascular endothelial growth factor (VEGF) levels are high,br /
but causing vessel regression when VEGF levels are low. Tie-1 is a Tie-2 homologue but itsbr /
ligands are unknown. Angiopoietin and Tie genes are expressed in the mammalianbr /
metanephros, the precursor of the adult kidney, where they may play a role in endothelialbr /
precursor growth. Tie-1-expressing cells can be detected in the metanephros when it firstbr /
forms and, based on transplantation experiments, these precursors contribute to the generationbr /
of glomerular capillaries. During glomerular maturation, podocyte-derived Ang-2 andbr /
mesangial-cell-derived Ang-2 may affect growth of nascent capillaries.br /
After birth, vasa rectae acquire their mature configuration and Ang-2 expressed by descendingbr /
limbs of loops of Henle would be well placed to affect the growth of this medullarybr /
microcirculation. Finally, preliminary data implicate angiopoietins in deregulated vesselbr /
growth in Wilms' kidney tumours and in vascular remodelling after nephrotoxicity. Altogether,br /
existing data suggest that VEGF-A and Angiopoietins not only have quite differentbr /
but also very complementary and coordinated roles.br /
SOURCE: Insect cellsbr /
PURITY: Greater than 95.0% as determined by:br /
(a) Analysis by RP-HPLC.br /
(b) Anion-exchange FPLCbr /
DIMERS amp; AGGREGATES: lt;1% as determined by silver stained SDS-PAGE gel analysisbr /
ENDOTOXIN: Less than 0.1 ng/mu;g (IEU/mu;g) of rhANG-2br /
PROTEIN CONTENT: Protein quantitation was carried out by two independent methods:br /
1) UV spectroscopy at 280 nm.br /
2) Analysis by RP-HPLC, using a standard solution of ANG1 as a Reference Standard.br /
FORM: Purifiedbr /
STORAGE: -20deg;C, avoid freezing and thawing cycles after reconstitutionbr /
BIOLOGICAL ACTIVITY: rhANG-2 is fully biologically active when compared to standard./p
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