Aeromonas Aminopeptidase (AP)

pDESCRIPTION: Recombinant Aeromonas Aminopeptidase (rAP)br /
The Enzyme (29 kDa) Aeromonas Aminopeptidase is used in the processing of pharmaceutical proteins produces by genetic engineering as well as forbr /
physical and structural investigations and for sequence and amino-terminalbr /
determinations. This exopeptidase recognizes a specific stop sign at ndash;X- Probr /
and requires a free a-amino group in the L-configuration. It is therefore suitablebr /
for the removal of the redundant N-terminal methionine often added to engineered recombinant proteins.br /
SOURCE: Aeromonas proteolytica expression systembr /
PURITY: gt; 98%, as determined by:br /
(a) Analysis by RP-HPLCbr /
(b) Analysis by reducing and non-reducing SDS-PAGE Silverbr /
Stained gelbr /
UNIT DEFINITION: 120 Units/mg protein. One unit is defined as the amount of enzyme that hydrolyses 1mu;mole of L-leucine p-nitroanilide at 25deg;C per 1 minute.br /
FORM: Purified, in 2-5 mg/ml in 10mM Tris-HCL, 100mM NaCl, 5mu;M ZnSO4, pH 8.0.br /
STORAGE: -20deg;C (aliquot), avoid freezing and thawing cycles/p