Acid Phosphatase1 (Acid phosphatase 1, soluble isoform b, His-tagged), Human

pDESCRIPTION: Acid phosphatase 1, soluble, also known as ACP1, belongs to the phosphotyrosine protein. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate.br /
Synonyms: ACP1, HAAP, LMW-PTP, Red cell acid phosphatase 1, Adipocyte acid phosphatasebr /
AA Sequence:br /
MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENWbr /
VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDLbr /
NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAHbr /
15% SDS-PAGE (3ug)br /
SOURCE: Recombinant, His-tagged (N),E.coli expression systembr /
MOLECULAR WEIGHT: 20.1 kDa (178 aa)br /
PURITY: gt; 95% by SDS PAGEbr /
FORM: Purified, in 20 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA, 10% glycerolbr /
SPECIFIC ACTIVITY: gt; 60,000 unit/mg of proteinbr /
UNIT DEFINITION: One unit is defined as the amount of enzyme that will hydrolyze 1nmole of p-nitrophenyl phosphate per minute at 37℃ in MES pH5.0 using 10mM of substratebr /
ENDOTOXIN: lt; 1.0 EU per 1 mu;g of protein (determined by LAL method )br /
CONCENTRATION: 1 mg/ml (Bradford Assay)br /
STORAGE: -20deg;C (aliquot), Avoid repeated freeze and thaw cycles/p