VECTREX Avidin DLA Reversible Binding and Elution Kit

For Biotin Labeled Nucleic Acids



VECTREX^® Avidin DLA (MB-2021) is used for reversible binding of biotinylated nucleic acids. VECTREX^® Avidin DLA is made by coupling our VECTREX^® matrix to a modified form of Avidin D that has a significantly reduced binding affinity for biotin. The affinity is such that, unlike VECTREX^® Avidin D, biotinylated protein, nucleic acids, or other molecules can be eluted with biotin. The matrix is supplied as a 1:1 suspension in buffer. The binding capacity is at least 25 ng of biotin-labeled λ DNA per μl of 1:1 suspension.



The VECTREX^® matrix is optimized for immobilization of nucleic acids. This matrix consists of particles of a highly cross-linked sugar polymer that has a very large surface area and low nonspecific binding for nucleic acids. Unlike agarose, which may retain small molecules in its pores, the VECTREX^® particle will retain molecules only through specific affinity interaction on the surface of the particles. The VECTREX^® matrix is dense and sediments readily even without centrifugation.



VECTREX^® Avidin DLA Binding and Elution Kit



VECTREX^® Avidin DLA Binding and Elution Kit (MB-1397) contains 1 ml of settled VECTREX^® Avidin DLA, Binding buffer and biotin solution for elution.