VECTREX Avidin D Irreversible Nucleic Acid Binding Matrix

VECTREX^® Avidin D (A-2020) is used for irreversible binding of biotinylated nucleic acids. This product consists of Avidin D covalently linked to the surface of the VECTREX^® matrix.



The VECTREX^® matrix is optimized for immobilization of nucleic acids. This matrix consists of particles of a highly cross-linked sugar polymer that has a very large surface area and low nonspecific binding for nucleic acids. Unlike agarose, which may retain small molecules in its pores, the VECTREX^® particle will retain molecules only through specific affinity interaction on the surface of the particles. The VECTREX^® matrix is dense and sediments readily even without centrifugation.



VECTREX^® Avidin D is useful in applications such as subtractive hybridization. A probe that is biotinylated with PHOTOPROBE^®, FastTag^®, or 3’ or 5’ EndTag™ Labeling Systems is hybridized to a mixture of nucleic acid in solution. After hybridization, the mixture is incubated with a suspension of VECTREX^® Avidin D or passed through a column containing VECTREX^® Avidin D. The DNA or RNA that is not associated with the biotinylated probe is left in solution or passes through the column. The biotinylated probe and the nucleic acid complementary to the probe are thus separated from the noncomplementary sequences. This matrix is supplied as a 1:1 suspension in buffer. The binding capacity is at least 22 ng of biotin-labeled λ DNA per μl of 1:1 suspension.