VECTREX AAL Reversible Binding and Elution Kit
- 貨號 Cat. No.
- MB-1397
- 規格 Size
- 1 kit
- 品牌 Brand
- VECTOR Labs
Description
| For Fucose Labeled Nucleic Acids VECTREX® AAL is used for reversible binding of fucose-labeled nucleic acids. Binding and elution can be performed at physiological pH and salt concentrations. VECTREX® AAL contains Aleuria aurantia lectin and will bind nucleic acids labeled with fucose using either the FastTag® system, or 3’ or 5’ EndTag™ Labeling Systems. Elution is easily accomplished under non-denaturing conditions with L-fucose. The matrix is supplied as a 1:1 suspension in buffer. The binding capacity is at least 25 ng fucose-labeled λ DNA per μl 1:1 suspension. The VECTREX® matrix is optimized for immobilization of nucleic acids. This matrix consists of particles of a highly cross-linked sugar polymer that has a very large surface area and low nonspecific binding for nucleic acids. Unlike agarose, which may retain small molecules in its pores, the VECTREX® particle will retain molecules only through specific affinity interaction on the surface of the particles. The VECTREX® matrix is dense and sediments readily even without centrifugation. |
| The Aleuria aurantia lectin conjugated to VECTREX® particles reversibly binds fucose. Thus fucosylated nucleic acids or other molecules can be bound to VECTREX® AAL under a wide variety of conditions and subsequently eluted with fucose under non-denaturing conditions. Potential applications include probe purification, PCR, cDNA and subtraction library construction, hybridization analysis, and in vitro mutagenesis. VECTREX® AAL Binding and Elution Kit (MB-1397) contains 1 ml of hydrated VECTREX® AAL, binding buffer, and L-fucose elution solution. |
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| Reversible binding of FastTag™ Fucose-labeled l Hind III DNA to VECTREX® AAL. Labeled (+) or unlabeled (-) DNA was incubated with VECTREX® AAL (+) or binding buffer (-). Following centrifugation of binding reactions, VECTREX® AAL was washed and the bound DNA eluted with L-fucose. Supernatants and eluates from the binding reactions were fractionated by agarose gel electrophoresis and DNA visualized by ethidium bromide staining. |
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