VECTASTAIN ABC-AmP Reagent for Western Blot Detection (Standard Kit)
- 貨號 Cat. No.
- AK-6000
- 規格 Size
- 1 kit
- 品牌 Brand
- VECTOR Labs
Description
| Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background. The VECTASTAIN® ABC-AmP™ is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent/ chemifluorescent or a chromogenic substrate. Used with the DuoLuX™ Chemiluminescent/Chemifluorescent Substrate, the VECTASTAIN® ABC- AmP™ system produces a very high and sustained light emission signal, with low background, and permanent fluorescence. The sensitivity of VECTASTAIN® ABC-AmP™ Reagent combined with the DuoLux™ substrate allows for the detection of as little as 1 pg of target protein. The VECTASTAIN® ABC-AmP™ Reagent is sufficient to develop approximately twenty 100 cm2 blots. |
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| Western blot visualized by using the VECTASTAIN® ABC-AmP™ kits. Serial dilutions (1:2) of maltose binding protein (MBP) were resolved by electrophoresis on a 12% PAGE reducing gel, transferred onto nitrocellulose membrane and detected with biotinylated anti-MBP made in goat, followed by the VECTASTAIN® ABC-AmP™ Reagent. Blots were developed with [A] DuoLuX™ Substrate using chemiluminescence, or [B] BCIP/NBT substrate. |
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