Unfractionated Heparin (UFH) ELISA for Plasma
Kits
- 貨號 Cat. No.
- K-2100
- 規格 Size
- 96 wells
- 品牌 Brand
- Lifespan Technologies
- Sample Type
- Plasma
Description
Heparin is a glycosaminoglycan with alternating uronic acid and aminoglycoside units. It is an anticoagulant used either in its native
unfractionated form (UFH) MW ~16 kD or in various partially depolymerized forms (LMWH) of 4-8 kD. The heparin-ELISA
product number K-2100 is a quantitative enzyme-linked assay designed for the in vitro measurement of unfractionated heparin levels
in plasma. This assay measures heparin directly using a heparin binding protein which has been conjugated to HRP.
The heparin-ELISA is a competitive assay in which the colorimetric signal is inversely proportional to the amount of heparin present
in the sample. Samples to be assayed are first mixed with the Detector-Enzyme Conjugate in wells of the heparin coated plate.
Heparin in the sample competes with heparin bound to the plate for binding of the Detector-Enzyme Conjugate. The concentration of
heparin in the sample is determined using a standard curve of known amounts of heparin. For best results, the heparin used for the
standard curve should match the type of heparin being assayed.
unfractionated form (UFH) MW ~16 kD or in various partially depolymerized forms (LMWH) of 4-8 kD. The heparin-ELISA
product number K-2100 is a quantitative enzyme-linked assay designed for the in vitro measurement of unfractionated heparin levels
in plasma. This assay measures heparin directly using a heparin binding protein which has been conjugated to HRP.
The heparin-ELISA is a competitive assay in which the colorimetric signal is inversely proportional to the amount of heparin present
in the sample. Samples to be assayed are first mixed with the Detector-Enzyme Conjugate in wells of the heparin coated plate.
Heparin in the sample competes with heparin bound to the plate for binding of the Detector-Enzyme Conjugate. The concentration of
heparin in the sample is determined using a standard curve of known amounts of heparin. For best results, the heparin used for the
standard curve should match the type of heparin being assayed.
