Unfractionated Heparin (UFH) ELISA for Buffer

Heparin is a glycosaminoglycan with alternating uronic acid and aminoglycoside units. It is an anticoagulant used either in its native
unfractionated form (UFH) MW ~16 kD or in various partially depolymerized forms (LMWH) of 4-8 kD. The heparin-ELISA
product number K-1900 is a quantitative enzyme-linked assay designed for the in vitro measurement of unfractionated heparin levels
in low protein content fluids such as buffer or urine. This assay measures heparin directly using a heparin binding protein which has
been conjugated to HRP.
The heparin-ELISA is a competitive assay in which the colorimetric signal is inversely proportional to the amount of heparin present
in the sample. Samples to be assayed are first mixed with the Detector-Enzyme Conjugate in wells of the heparin coated plate.
Heparin in the sample competes with heparin bound to the plate for binding of the Detector-Enzyme Conjugate. The concentration of
heparin in the sample is determined using a standard curve of known amounts of heparin.