Toxoplasma IgA ELISA
- 貨號 Cat. No.
- TX023A
- 規格 Size
- 96 Tests
- 品牌 Brand
- Calbiotech
Description
Toxoplasma IgA ELISA
Category Name
Infectious Diseases
Method
ELISA
Principle
ELISA
Controls Standards
Positive, Negative and Calibrator
Sensitivity
Not available
Sample
100 µl
Runtime
50 Minutes
Wavelength
450 nm
Safety
Please refer to MSDS
Shelflife
18 Months
INTENDED USE
The Calbiotech Toxoplasma IgA ELISA Kit is intended for the detection of IgA antibody to Toxoplasma in human serum or plasma.
SUMMARY AND EXPLANATION
Toxoplasma gondii causes toxoplasmosis, a common disease that affects 30-50 of every 100 people in North America by the time they are adults. The mean source of infection is direct contact with cat feces or from eating undercooked meats. Toxoplasmosis generally presents with mild symptoms in immunocompetent individuals; in the immunocompromised patient, however, the infection can have serious consequences. Acute toxoplasmosis in pregnant women can result in result in miscarriage, poor growth, early delivery or stillbirth. Treatment of an infected pregnant woman may prevent or lessen the disease in her unborn child. Treatment of an infected infant will also lessen the severity of the disease as the child grows. IgG and IgM antibodies to Toxoplasma can be detected with 2-3 weeks after exposure. IgG remains positive, but the antibody level drops overtime. ELISA can detect Toxoplasma IgM antibody after one year after infection in over 50% of patients. Therefore, IgM positive results should be evaluated further with one or two follow up samples if primary infection is suspected.
PRINCIPLE OF THE TEST
Diluted patient serum is added to wells coated with purified Toxoplasma antigen. Toxoplasma IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgA specific antibody in the sample.
