T4 Total ELISA
- 貨號 Cat. No.
- T4044T
- 規格 Size
- 96 Tests
- 品牌 Brand
- Calbiotech
Description
T4 Total ELISA
Category Name
Thyroid Markers
Method
ELISA
Principle
ELISA
Controls Standards
0,2.0,5.0,10.0,15.0,25.0 µg/dl
Sensitivity
0.4µg/dl
Sample
50 µl
Runtime
75 min
Wavelength
450 nm
Safety
Please refer to MSDS
Shelflife
18 Months
INTENDED USE
The Calbiotech Thyroxine (T4) ELISA Kit is intended for the detection of Total T4 in human serum or plasma.
Summary and explanation
T4 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T4 is decreased in hypothyroid patients and is increased in hyperthyroid patients. The level of T4 is normal in Euthyroid individuals.
PRINCIPLE OF THE TEST
The CBI T4 is a solid phases competitive ELISA. The samples, assay buffer and T4 enzyme conjugate are added to the wells coated with anti-T4 monoclonal antibody. T4 in the patient’s serum competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound T4 and T4 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is prepared relating color intensity to the concentration of the T4.
