T3 Total ELISA
- 貨號 Cat. No.
- T3043T
- 規格 Size
- 96 Tests
- 品牌 Brand
- Calbiotech
Description
T3 Total ELISA
Category Name
Thyroid Markers
Method
ELISA
Principle
ELISA
Controls Standards
0,0.5,1.0,2.5,5.0,7.5 ng/ml
Sensitivity
0.04ng/ml
Sample
50 µl
Runtime
75 min
Wavelength
450 nm
Safety
Please refer to MSDS
Shelflife
18 Months
INTENDED USE
The Calbiotech Triiodothyronine (T3) ELISA Kit is intended for the quantitative measurement of Triiodothyronine (T3) in human serum or plasma.
Summary and explanation
Triiodothyronine (T3) is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T3 is decreased in hypothyroid patients and is increased in hyperthyroid patients, graves disease and pregnancy.
PRINCIPLE OF THE TEST
The CBI T3 is a solid phases competitive ELISA. The samples, assay buffer and T3 enzyme conjugate are added to the wells coated with anti-T3 monoclonal antibody. T3 in the patient’s serum competes with a T3 enzyme conjugate for binding sites. Unbound T3 and T3 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve is prepared relating color intensity to the concentration of the T3.
