ProtOn Biotin Protein Labeling Kit

Protein labeling and modification is a powerful tool for the isolation and analysis of proteins. The ProtOn™ Protein Labeling Kits simplify the attachment of either biotin or fluorescein to antibodies or other proteins. Vector Laboratories offers a variety of reagents and kits for protein labeling allowing for greater flexibility of experimental design.



This ProtOn™ protein labeling kit is designed for the simple and fast labeling of proteins with biotin. The kit is ideally suited to label antibodies, hormones, or other proteins as well as other macromolecules containing primary amino groups. Labeled proteins can be used in a number of applications, such as immunological assays, fluorescence microscopy, investigation of protein-protein interactions, etc.



The ProtOn™ labeling reagent reacts with primary amines (i.e. lysine residues or terminal amino groups) on the protein forming a stable covalent bond. Each kit contains all the components needed for at least five labeling reactions (up to 1 mg of protein per reaction):

• Labeling Reagent (Biotin [Long Arm] NHS)


• Reagent Solvent


• Reaction Buffer


• Stop Reagent


• Gel Filtration Slurry


• 5 x 1 ml Spin Columns


• 5 x 2 ml Collection Tubes


• Agarose Avidin D Slurry**