Prestained Protein Marker

   

Prestained Protein Marker

Cat. No. abs-71                                                                                                                                                         Size: 500µl

 

 

l  Broad range: 15-175 kDa.

l  Ready-to-use: supplied in a loading buffer for direct loading on gels.

l  lncludes the 15 and kDa reference bands of enhanced intensity.

l  Clear bands.

 

 

Description

Prestained Protein Marker (abs-71), is a mixture of purified proteins covalently coupled to a blue dye that resolves to 9 bands when electrophoresed. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS- PAGE gels relative to unstained proteins. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system.

 

＊The intensity for the reference bands of 15 and 75 kDa is particularly

 

 

Applications

l  Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.

l  Monitoring of protein transfer onto membranes during Western blotting.

l  Sizing of proteins on SDS-polyacrylamide gels and Western blots.

 

 

Quality Control

Tested in SDS-polyacrylamide gel electrophoresis and Western blots.

 

 

Storage

Store at -20℃.

Stable at +4℃ for 3 months. 

 

 

Recommendations for Loading

1. Thaw the ladder either at room temperature or at 37-40℃ for a few minutes to dissolve precipitated solids. Do not boil.

2. Mix thoroughly, to ensure that the solution is homogeneous..

3. Load the following volumes of the ladder on SDS-polyacrylamide gel:

- 10 µl per well for mini-gels, 6 µl per well for blots;

- 20 µl per well for large gels, 12 µl per well for blots.