10 mM dNTP Mix
- 貨號 Cat. No.
- abs-61
- 規格 Size
- 1 ml
- 品牌 Brand
- abs
Description
10 mM dNTP Mix
Catalog No. abs-61 Size 1 ml
Store at -20°
Description
10 mM dNTP (2’-deoxynucleoside 5’-triphosphate) Mix consists of all four nucleotides (dATP, dCTP, dGPT, dTTP), each at a concentration of 10 mM, in a solution of 0.6 mM Tris-HCI (pH 7.5). The 10 mM dNTP Mix is suitable for use polymerase chain reaction (PCR), sequencing, fill-in, nick translation, cDNA synthesis, and TdT-tailing reactions.
Quality Control
10 mM dNPT Mix is tested in a standard procedure to synthesize first-strand cDNA and is evaluated in a functional assay for PCR.
Volumes and Concentrations
20 μl Final Reaction Volume
Final dNTP Conc | dNTP Volume | 1 ml Kit |
0.2 mM | 0.4 μl | 2,500 |
0.5 mM | 1 μl | 1,000 |
1.0 mM | 2 μl | 500 |
1.5 mM | 3 μl | 333 |
25 μl Final Reaction Volume
Final dNTP Conc | dNTP Volume | 1 ml Kit |
0.2 mM | 0.5 μl | 2,000 |
0.5 mM | 1.25 μl | 1,000 |
1.0 mM | 2.5 μl | 800 |
1.5 mM | 3.75 μl | 266 |
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