NEUROBIOTIN-Plus

Investigators have identified rapid degradation of injected tracers such as biocytin due to the presence of biotinidase activity in brain tissue*. This loss of stability of the tracer significantly reduces the viable postinjection period and may compromise complete detection of a neural network. NEUROBIOTIN™-Plus was designed to be impervious to cleavage and breakdown by biotinidase. This newly synthesized biotin-containing compound contains fixable amine groups, is highly soluble and has a low molecular weight. These characteristics make it an ideal candidate for experiments requiring long postinjection survival times and optimal uptake along the entire neural tract.



NEUROBIOTIN™-Plus shares the same key advantages of NEUROBIOTIN™ Tracer over biocytin and other neuronal labels:

• Better solubility


• More efficiently iontophoresed


• Remains in cell longer


• Non-toxic


• Can be fixed with formalin or glutaraldehyde
  
  
  
  NEUROBIOTIN™-Plus can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods.
  
  
  
  *Mishra, A. et al (2010) ACS Chem. Neurosci. 1:129-138.