Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
- Clear, crisp specific staining of antigens of interest
- Simple, easy procedure
- No tedious calculations required
- Non-biotin
A Vector® M.O.M.™ Kit is now available containing our proprietary M.O.M.™ Mouse Ig Blocking Reagent paired with a specialized, highly sensitive, ready-to-use, one-step, non-biotin M.O.M.™ ImmPRESS™ peroxidase polymer reagent. Producing the same outstanding staining as the original M.O.M.™ Peroxidase Kit is now even easier using the M.O.M.™ ImmPRESS™ Peroxidase Polymer Kit.
For years, researchers attempting to localize mouse primary antibodies on mouse tissue have relied on the Vector® M.O.M.™ Kit to produce crisp, strong, specific staining of antibody targets. The specific detection of mouse primary antibodies on mouse tissue is difficult due to the presence of endogenous mouse immunoglobulins. A conventional anti-mouse detection system cannot distinguish between the mouse primary antibody and the endogenous mouse immunoglobulins leading to high background. The Vector® M.O.M.™ Kit, including a proprietary M.O.M.™ Mouse Ig Blocking Reagent and a paired, specialized secondary antibody detection system, essentially eliminates this problem by significantly reducing undesired binding of the secondary antibody to endogenous tissue immunoglobulin.
The Vector® M.O.M.™ ImmPRESS™ Kit (Cat. No. MP-2400) contains sufficient reagents to stain approximately 150 mouse sections and includes the following reagents:
- 0.6ml M.O.M.™ Mouse Ig Blocking Reagent (concentrate)
- 30 ml R.T.U. 2.5% Normal Horse Serum
- 15 ml R.T.U. M.O.M.™ ImmPRESS™ Anti-Mouse Ig Reagent
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