Mouse on Mouse (M.O.M.) Blocking Reagent

The Vector^® M.O.M.™ Immunodetection Kits are specifically designed to localize mouse primary antibodies on mouse tissues. The major problem with using mouse primary antibodies on mouse tissues in immunohistochemistry is the inability of the anti-mouse secondary antibody to distinguish between the mouse primary antibody and endogenous mouse immunoglobulins in the tissue. This can result in high background staining which obscures specific staining. This problem can be essentially eliminated by using a Vector^® M.O.M.™ Immunodetection Kit which includes a proprietary M.O.M.™ Mouse Ig Blocking Reagent with a specialized M.O.M.™ Biotinylated Anti-Mouse Ig to significantly reduce undesired binding of the secondary antibody to endogenous tissue immunoglobulin.



Key advantages of the Vector^® M.O.M.™ Immunodetection Kits:

• Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue


• Clear, crisp specific staining of antigens of interest


• Based on VECTASTAIN^® ABC reagent protocols


• Procedures are simple and easy to follow


• No tedious calculations or antibody prebinding steps required
  
  
  
  The Vector^® M.O.M.™ Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts.
  
  
  
  The M.O.M.™ Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems. Detailed information for multiple antigen labeling can be found in our brochure, “ Discovery Through Color: A Guide to Multiple Antigen Labeling”, available on our website.
  
  
  
  Three Vector^® M.O.M.™ Kits are available. All kits use the same proprietary M.O.M.™ Mouse Ig Blocking Reagent and the M.O.M.™ Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M.™ Mouse Ig Blocking Reagent and M.O.M.™ Biotinylated Anti-Mouse Ig Reagent are also available separately.
  
  
  
  M.O.M.™ Mouse Ig Blocking Reagent (MKB-2213) is a key component of the M.O.M.™ Kits and is used to block endogenous mouse antibody in the tissue section. Optimal staining results are obtained when this reagent is paired with the special M.O.M.™ Biotinylated Anti-Mouse Ig Reagent. However, if a biotin-free detection system is preferred, the M.O.M.™ Blocking Reagent can be used with the ImmPRESS™ Anti-Mouse Ig Kit (MP-7402).
  
  
  
  This reagent is supplied as a 1 ml concentrate, sufficient to prepare about 25 ml of working solution or enough to stain about 125-250 sections.