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Mouse on Mouse (M.O.M.) Basic Kit

貨號 Cat. No.
BMK-2202
規格 Size
1 kit
品牌 Brand
VECTOR Labs

Description























The Vector® M.O.M.™ Immunodetection Kits are specifically designed to localize mouse primary antibodies on mouse tissues. The major problem with using mouse primary antibodies on mouse tissues in immunohistochemistry is the inability of the anti-mouse secondary antibody to distinguish between the mouse primary antibody and endogenous mouse immunoglobulins in the tissue. This can result in high background staining which obscures specific staining. This problem can be essentially eliminated by using a Vector® M.O.M.™ Immunodetection Kit which includes a proprietary M.O.M.™ Mouse Ig Blocking Reagent with a specialized M.O.M.™ Biotinylated Anti-Mouse Ig to significantly reduce undesired binding of the secondary antibody to endogenous tissue immunoglobulin.



Key advantages of the Vector® M.O.M.™ Immunodetection Kits:




  • Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue

  • Clear, crisp specific staining of antigens of interest

  • Based on VECTASTAIN® ABC reagent protocols

  • Procedures are simple and easy to follow

  • No tedious calculations or antibody prebinding steps required



    The Vector® M.O.M.™ Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts.



    The M.O.M.™ Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems. Detailed information for multiple antigen labeling can be found in our brochure, “ Discovery Through Color: A Guide to Multiple Antigen Labeling”, available on our website.



    Three Vector® M.O.M.™ Kits are available. All kits use the same proprietary M.O.M.™ Mouse Ig Blocking Reagent and the M.O.M.™ Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M.™ Mouse Ig Blocking Reagent and M.O.M.™ Biotinylated Anti-Mouse Ig Reagent are also available separately.
Vector® M.O.M.™ Basic Kit (BMK-2202) includes the following:

  • M.O.M.™ Mouse Ig Blocking Reagent

  • M.O.M.™ Biotinylated Anti-Mouse Ig Reagent

  • M.O.M.™ Protein Concentrate for general protein blocking and diluent



    An enzyme or fluorochrome conjugate is not included allowing for a choice of any avidin or streptavidin-based detection system. Choose any peroxidase or alkaline phosphatase conjugated avidin or streptavidin for chromogenic detection (for example, VECTASTAIN® ABC-AP Standard Kit followed by a Vector® alkaline phosphatase substrate), or choose a fluorochrome-labeled avidin or streptavidin.
This Vector® M.O.M.™ kit contains sufficient stock reagents to provide approximately 25 ml of working solution, which is usually enough to stain about 125-250 mouse tissue sections.
Sections of mouse kidney stained with mouse antibody against smooth muscle actin using VECTASTAIN® ABC-AP reagents and Vector® Blue substrate. Left photo: Using standard biotinylated anti-mouse antibody and normal blocking serum, confusing background interference is seen. Right photo: With the Vector® M.O.M.™ Basic kit, clean background and specific staining is achieved.
In addition, the Vector® M.O.M.™ Basic kit can be used to localize two different mouse primary antibodies in the same mouse tissue section. By using a multiple antigen staining protocol, two fluorescent avidin labels or two enzyme-based stains can be introduced on one section.

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