ImmEdge Hydrophobic Barrier Pen

The ImmEdge™ Pen (H-4000) is an improvement over the PAP pen for immunohistochemistry and in situ hybridization. It is designed to provide a heat-stable, water-repellent barrier that keeps reagents localized on tissue specimens and prevents mixing of reagents when multiple sections are mounted on the same slide.



The pale blue barrier is insoluble in alcohol and acetone but is completely removed by all commonly used xylene and “xylene substitute” clearing agents. The ImmEdge™ Pen is compatible with enzyme or fluorescence-based detection systems.



The ImmEdge™ Pen is environmentally friendly - it does not contain "ozone-depleting" solvents as identified by the Montreal Protocol or the Council of the European Union (specifically, fluorocarbons and chlorocarbons).