hCG ELISA
- 貨號 Cat. No.
- HC047F
- 規格 Size
- 96 Tests
- 品牌 Brand
- Calbiotech
Description
hCG ELISA
Category Name
Fertility Markers
Method
ELISA
Principle
ELISA
Controls Standards
0,10,25,50,100,250mIU/ml
Sensitivity
0.53mIU/ml
Sample
50 µl
Runtime
40 min
Wavelength
450 nm
Safety
Please refer to MSDS
Shelflife
18 Months
INTENDED USE
The Calbiotech hCG ELISA Kit is intended for the quantitative measurement of hCG in human serum or plasma.
SUMMARY AND EXPLANATION
Human Chorionic Gonadotropin (hCG) is a 40 kD glycoprotein hormone secreted by the placenta. hCG has two subunits, alpha and beta. The alpha subunit is similar to the alpha subunit found in LH, FSH and TSH glycoprotein hormones. However, the beta subunit is specific and differs from hormone to hormone.
The serum hCG rises in early pregnancy to concentrations of 50,000-150,000 mIU/ml between the 8th and 12th weeks of gestation and decline to 20,000 mIU/ml by the 18th week where they remain for the duration of the pregnancy. The increased level of hCG in non-pregnant women or men suggest neoplsia. Thus hCG measurement is useful for the recognition and monitoring of chorionic tumors and as a tumor marker for other malignancies that produce hCG ectopically. These include testicular, pancreatic and bronchogenic pulmonary cancers. The sensitivity of this ELISA test is 0.5mIU/ml.
PRINCIPLE OF THE TEST
The CBI hCG is a direct solid phase sandwich ELISA method. The samples and diluted anti-hCG-HRP conjugate are added to the wells coated with Mab to beta subunit. hCG in the patient’s serum binds to anti-hCG MAb on the well and the anti-hCG second antibody then binds to hCG. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of hCG in the samples. A standard curve is prepared relating color intensity to the concentration of the hCG.
