Fluorescein labeled Euonymus Europaeus Lectin (EEL)

EEL consists of six closely related lectins with isoelectric points between pH 4.3 and pH 4.7. Most of the 35 kDa subunits appear to consist of two disulfide linked chains of about 17 kDa. This lectin has a carbohydrate binding specificity toward type 1 or type 2 chain blood group B structures but will bind other oligosaccharides containing galactosyl (α-1,3) galactose. Unlike Ulex europaeus and Lotus tetragonolobus lectins, EEL has a high affinity toward type 1 chain blood group H structures. This lectin has been reported to bind to endothelial cells from human and non-human sources and recognizes carbohydrate structures on the surface of stimulated murine peritoneal lymphoid cells.



Fluorescein labeled Euonymus europaeus lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.



Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.



Inhibiting/Eluting Sugar: 500 mM lactose