Estradiol lumELISA
- 貨號 Cat. No.
- ES561S
- 規格 Size
- 96 Tests
- 品牌 Brand
- Calbiotech
Description
Estradiol lumELISA
Category Name
Steroids
Method
Chemiluminescent ELISA
Principle
Chemiluminescent ELISA
Controls Standards
Sensitivity
Sample
Runtime
Wavelength
Safety
Please refer to MSDS
Shelflife
18 Months
INTENDED USE
The Calbiotech, Inc. (CBI) Ultra Sensitive Estradiol (E2) lumELISA (Chemiluminescence Enzyme Linked Immunosorbent Assay) is used for the ultra sensitive quantitative measurement of Estradiol (E2) in human serum or plasma.
SUMMARY AND EXPLANATION OFTHE TEST
Estradiol (E2) is a C18 steroid hormone with a phenolic A ring. This steroid hormone has a molecular weight of 272.4. Estradiol E2 is the major bioactive Estrogen, produced mainly by the ovary, placenta, and in smaller amounts by the adrenal cortex, and the male testes. Estradiol is secreted into the blood stream where 98% bound to sex hormone binding globulin (SHBG). Estrogenic activity is effected via estradiol-receptor complexes which trigger the appropriate response at the follicles, uterus, breast, vagina, urethra, hypothalamus, pituitary and to a lesser extent the liver and skin. In non-pregnant women with normal menstrual cycles, estradiol secretion follows a cyclic, biphasic pattern with the highest concentration found immediately prior to ovulation. The rising estradiol concentration exert a positive feedback influence at the level of the pituitary where it influences the secretion of the gonadotropins, follicle stimulating hormone, and luteinizing hormone, which are essential for follicular maturation and ovulation, respectively. Following ovulation, estradiol levels fall rapidly until the luteal cells become active resulting in a secondary gentle rise and plateau of estradiol in the luteal phase. During pregnancy, maternal serum Estradiol levels increase considerably, to well above the pre-ovulatory peak levels and high levels are sustained throughout pregnancy. Serum Estradiol measurements are a valuable index in evaluating a variety of menstrual dysfunctions such as precocious or delayed puberty in girls and primary and secondary amenorrhea and menopause. Estradiol levels have been reported to be increased in patients with feminizing syndromes, gynaecomastia and testicular tumors. In cases of infertility, serum Estradiol measurements are useful for monitoring induction of ovulation following treatment.Estradiol EIA kits are designed for the measurement of total Estradiol in human serum or plasma. The assay has high sensitivity well suited to measurements in children and to diagnosis of the menopause.
PRINCIPLE OF THE TEST
The CBI E2 sensitive lumELISA kit is based on the principle of competitive binding between E2 in the test specimen and E2 Enzyme conjugate for a constant amount of rabbit anti-Estradiol antibody. E2 standards, controls, patient samples, Estradiol Enzyme Conjugate and rabbit anti-Estradiol antibody are incubated in Goat anti-rabbit IgG-coated wells. During the incubation, a fixed amount of Enzyme labeled E2 competes with the endogenous E2 in the standard, sample, or quality control serum for a fixed number of binding sites of the specific E2 antibody. E2 peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of E2 in the specimen increases. Unbound E2 peroxidase conjugate is then removed and the wells washed.Upon the addition of the luminol substrate, the enzyme activity in the enzyme-bound fraction is inversely proportional to the concentration of the E2 in the samples. A standard is prepared relating light unit (RLU) to the concentration of the E2.
