DuoLuX Chemiluminescent and Fluorescent Alkaline Phosphatase Substrate
- 貨號 Cat. No.
- SK-6605
- 規格 Size
- 100 ml
- 品牌 Brand
- VECTOR Labs
Description
The DuoLuX™ Chemiluminescent/Chemifluorescent Substrate is a unique formula based on acridan chemistry. The DuoLuX™ Substrate provides the following advantages:
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| Serial dilutions (1:2) of mouse IgG were resolved by PAGE, transferred onto nitrocellulose membrane and detected with the DuoLuX™ Substrate using either the Alkaline Phosphatase-based VECTASTAIN® ABC-AmP™ Kit (lower left) or Peroxidase Streptavidin (lower right). Graph shows prolonged light emission characteristics of the DuoLuXTM Substrate with each enzyme. |
| Fluorescent Properties In addition to its chemiluminescent properties, the reaction product of the DuoLuX™ Substrate is also fluorescent. Fluorescence can be recorded with a digital imaging system or a conventional camera months after chemiluminescence has faded. The excitation maximum is at 405 nm, but other wavelengths (254 nm and 365 nm) also excite. Maximum fluorescent emission occurs at 453 nm. For fluorescence detection, nitrocellulose is recommended due to the intrinsic fluorescence of nylon. Acquisition of fluorescent signal requires a much shorter exposure time than chemiluminescence, often a fraction of a second. |
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| Western blot visualized by fluorescence using the VECTASTAIN® ABCAmP™ Kit with the DuoLuX™ Substrate. Serial dilutions (1:2) of rabbit IgG heavy chain were resolved by PAGE, transferred onto nitrocellulose membrane, and detected with biotinylated anti-rabbit IgG, followed by the VECTASTAIN® ABC-AmP™ Kit with the DuoLuX™ Substrate. [A] Fluorescent detection by image acquisition (1 second). [B] Fluorescent image of the same blot as shown in [A] obtained 12 months later. (Same 1 second acquisition time.) |
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