Biotinylated Amaranthus Caudatus Lectin (ACL, ACA)
- 貨號 Cat. No.
- B-1255
- 規格 Size
- 2 mg
- 品牌 Brand
- VECTOR Labs
Description
| This lectin is a 66 kDa to 70 kDa dimer composed of two subunits of about 35 kDa. Four bands are resolved by isoelectric focusing. Unlike most lectins, ACL binds best in mildly acidic conditions. Although this lectin preferentially binds to oligosaccharides containing the galactosyl (β-1,3) N-acetylgalactosamine structure (T-antigen), tissue staining patterns are markedly different than those obtained with either peanut agglutinin or Jacalin. Amaranthus caudatus lectin also appears to tolerate sialic acid substitution at the 3 position of galactose in the “T” antigen. Biotinylated Amaranthus Caudatus Lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide. This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative. Inhibitor: Desialyzed fetuin or bovine submaxillary mucin |
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