ZO-1 Antibody (LS-B2228)

貨號 Cat. No.: LS-B2228
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2228
Antibody Validation:	This antibody replaces catalog number LS-C18587. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Tight Junction Protein Zo-1 (TJP1)
Synonyms:	TJP1, tight junction protein zo-1
Family:	Occludin
Subfamily:	Not assigned-Occludin
Host:	TJP1 antibody was produced in Mouse
Antigen Species:	TJP1 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1,k
Antigen Type:	Recombinant protein
Immunogen:	TJP1 antibody was raised against human recombinant ZO-1 fusion protein encompassing amino acids 334-634
Antibody Specificity:	Recognizes ZO-1a+ and ZO-1a- isoforms. Cross-reactivity with the related ZO-2 protein has not been observed. Species Cross-reactivity: Human and canine
Antigen Modification:	aa334-634
Predicted Species Reactivity:	Dog
Purification:	Protein A Column
Presentation:	PBS, pH 7.4, 0.1% sodium azide.
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2228 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2228 was determined to be 5 ug/ml. Western Blot: 1-2 ug/ml. Positive control: T84, Caco-2 and MDCK cells.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1 - 2 µg/ml), Immunofluorescence (IF) (15 - 20 µg/ml), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml