Antibodies
ZCL278
- 貨號 Cat. No.
- S7293
- 規格 Size
- 10mgã€50mg
- 品牌 Brand
- Selleckchem
Description
ZCL278
Catalog No.S7293
ZCL278 is a selective Cdc42 GTPase inhibitor with Kd of 11.4 μM.
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Distributor Tel: +886-2-2827-1197jimmy@new.abscience.com.tw
Biological Activity
| Description | ZCL278 is a selective Cdc42 GTPase inhibitor with Kd of 11.4 μM. | |||||
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| Targets | Cdc42 GTPase | |||||
| IC50 | 11.4 μM[1] | |||||
| In vitro | ZCL278 inhibits Cdc42 GTPase activity by the competition with GTP and inhibits Rac/Cdc42 phosphorylation in a time-dependent manner. ZCL278 (50 μM) inhibits Cdc42-mediated microspike formation and disrupts GM130-docked Golgi structures in serum-starved Swiss 3T3 fibroblasts. In addition, ZCL278 also suppresses Cdc42-mediated neuronal branching and growth cone dynamics as well as actin-based motility and migration in a metastatic prostate cancer PC-3 cell line without cytotoxicity. [1] | |||||
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| Features | ||||||
Protocol(Only for Reference)
Kinase Assay:
| p50RhoGAP or Cdc42GAP Assay | Inorganic phosphate produced as a result of GTPase activity is measured by using a p50RhoGAP or Cdc42GAP assay (Cytoskeleton) and absorbance-based detection method. Briefly, Cdc42 is preloaded with GTP or ZCL278 and incubated in the reaction buffer (provided in the kit) for 20 min at 37 °C. GAP is then added for an additional 20 min at 37 °C. Following a 10-min incubation in CytoPhos reagent (Cytoskeleton), inorganic phosphate was detected at 650 nm. |
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References
Chemical Information
| Molecular Weight (MW) | 584.89 |
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| Formula | C21H19BrClN5O4S2 |
| CAS No. | 587841-73-4 |
| Storage | 3 years -20℃Powder |
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| 6 months-80℃in DMSO | |
| Syonnyms | N/A |
| Solubility (25°C) * | In vitro | DMSO | 100 mg/mL (170 mM) |
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| Water | <1 mg/mL (<1 mM) | ||
| Ethanol | <1 mg/mL (<1 mM) | ||
| * <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. | |||
| Chemical Name | 2-(4-Bromo-2-chlorophenoxy)-N-[[[4-​[[(4,6-dimethyl-2-pyrimidinyl)amino]sulfonyl]pheny​l]amino]thioxomethyl]acetamide |
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