Z-Leu-Arg-Gly-Gly-AMC (Z-LRGG-AMC)
- 貨號 Cat. No.
- S-100
- 規格 Size
- 5 mg
- 品牌 Brand
- Boston Biochem
Description
Z-Leu-Arg-Gly-Gly-AMC (Z-LRGG-AMC)
human recombinant
Product Information
5 mg
11 kDa
> 95% by HPLC
Soluble in DMSO. For best results, pellet dry compound prior to reconstitution. Solubilize at desired stock concentration.
Use & Storage
Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM. (λex: 380nm; λem: 460 nm)
Store lyophilized powder or stock solution at -20°C. Avoid multiple freeze/thaw cycles.
Catalog #: S-100
Z-Leu-Arg-Gly-Gly-AMC (Z-LRGG-AMC)
Product Source:
human recombinant
Fluorgenic tetra-peptide substrate for ubiquitin C-terminal hydrolases (UCHs, eg. UCHL3) and Isopeptidase T. This low molecular weight peptide represents a minimal sequence of C-terminal residues of ubiquitin where hydrolysis occurs at the UbGly76-X bond. The catalytic efficiency for hydrolysis of this substrate by deconjugating enzymes is several thousand-fold less than a more native substrate (Ub-AMC, Cat # U-550) since remote interactions with Ub-containing substrates stabilize catalytic transition states.
Product Information
Quantity:
5 mg
MW:
11 kDa
Structure:
Purity:
> 95% by HPLC
Solubility:
Soluble in DMSO. For best results, pellet dry compound prior to reconstitution. Solubilize at desired stock concentration.
Western Blot Data:
Use & Storage
Use:
Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM. (λex: 380nm; λem: 460 nm)
Storage:
Store lyophilized powder or stock solution at -20°C. Avoid multiple freeze/thaw cycles.