YFP Antibody

Antibody Target: YFP

Alternative Name: Yellow Fluorescent Protein

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Recombinant YFP expressed from E. coli

Accession#: AAR85349

Gene ID: 40365351

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: all

Application: Western blot

Application & Usage: Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and immunofluorescence. However, the optimal conditions should be determined individually.

Storage Temp.: -20 °C

Shipping: gel pack

Shelf Life: 12 months

Background Descriptions: Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. YFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or YFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or YFP can fuse with proteins of interest without interfering significantly with their assembly and function.

Handling: The antibody solution should be gently mixed before use.