XAF-1 Antibody

Antibody Target: XAF-1

Alternative Name: XAF 1 , XIAP , HSXIAPAF1 , HSXIAPAF 1 , BIRC4 , BIRC 4

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Synthetic peptide surrounding a.a. 306 of human XAF-1

Accession#: CAA68030

Gene ID: 54739

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) Protein A affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: human

Application: Western blot

Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate (Cat.# 2401-100) can be used as the positive control. The antibody recognizes 34 kDa XAF-1 from samples of human origins. Reactivity to other species has not been tested.

Storage Temp.: -20 °C

Shipping: gel pack

Shelf Life: 12 months

Background Descriptions: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, -9 and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP’s ability to inhibit cell death. XAF-1is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines.

Handling: The antibody solution should be gently mixed before use.