Wnt-4 Antibody

Antibody Target: Wnt-4

Alternative Name: OTTHUMP00000044725 , WNT-4 , WNT-4 protein , WNT4

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Synthetic peptide surrounding amino acid 10 of human Wnt-4

Accession#: BAC23080

Gene ID: 54361

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: human, mouse, rat

Application: Western blot

Application & Usage: Western blotting (1-4 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate (Cat.# 2401-100) and mouse small intestine tissue lysate (Cat.# 2601-100) can be used as a positive controls. The antibody recognizes 39 kDa Wnt-4 of human, mouse and rat origins. Reactivity to other species has not been tested.

Storage Temp.: -20 °C

Shipping: gel pack

Shelf Life: 12 months

Background Descriptions: Wnt gene family members, including Wnt-1 through Wnt-10, play a key role in regulating cellular growth and differentiation. Wnt-1 is a cysteine-rich, secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. Wnt-3 is also involved in tumorigenesis and Wnt-2 and Wnt-4 may be associated with abnormal proliferation in human breast tissue.

Handling: The antibody solution should be gently mixed before use.