WNT-1, human recombinant

Alternate Names / Synonyms: INT-1

Gene Symbol: WNT1

Accession #: P04628

Gene ID: 7471

Gene Source: Human

Recombinant Host: E. coli

Appearance: Lyophilized protein

Physical Form: Sterile filtered and lyophilized with no additives

Molecular Weight: 38.4 kDa

Purity by SDS-PAGE: ≥98%

Purity by HPLC: ≥98%

Endotoxin Level: <0.1 ng/μg

Biological Activity: The EDâ‚…â‚€ was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected EDâ‚…â‚€ for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.

Reconstitution: Reconstitute in water to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers

Storage Temp.: -20°C

Shipping: Blue ice

Background Information: Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intraCellular signaling protein, β-catenine. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.

Handling: Centrifuge the vial prior to opening.