Wnt-1 Antibody

Antibody Target: Wnt-1

Alternative Name: wnt1, wnt 1, wnt-1, INT 1 , INT1, wingless type MMTV integration site family member 1

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Synthetic peptide surrounding amino acid 31 of human Wnt-1

Accession#: P04628

Gene ID: 7471

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit Wnt-1 polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: human

Application: Western blot

Application & Usage: Western blotting (0.5-4 µg/ml), Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 41kDa of human Wnt-1. Reactivity to other species has not been tested.

Storage Temp.: -20 °C

Shipping: gel pack

Shelf Life: 12 months

Background Descriptions: Wnt gene family members, including Wnt-1 through Wnt-10, play a key role in regulating cellular growth and differentiation. Wnt-1 is a cysteine-rich, secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. Wnt-1, which is essential for normal development of the embryonic nervous system, contributes to hyperplasia and tumorigenic progression when improperly expressed in mammary tissue. Wnt-3 is also involved in tumorigenesis and Wnt-2 and Wnt-4 may be associated with abnormal proliferation in human breast tissue. Wnt-1, Wnt-3 and Wnt-10b have been implicated along with FGF-3 in the development of mouse mammary tumor virus induced mouse mammary carcinomas.

Handling: The antibody solution should be gently mixed before use.