Wnt-1 Antibody

Antibody Target: Wnt-1

Alternative Name: wnt1, wnt 1, wnt-1, INT 1 , INT1, wingless type MMTV integration site family member 1

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: E. coli expressed human Wnt-1

Accession#: P04628

Gene ID: 7471

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-human Wnt-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: human

Application: Western blot

Application & Usage: Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human Wnt-1 (Cat.# 4754-10) can be used as a positive control.

Storage Temp.: -20 °C

Shipping: gel pack

Shelf Life: 12 months

Background Descriptions: Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenine. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues.