WNK1 (Y52) Antibody
- 貨號 Cat. No.
- BS1374
- 規格 Size
- 100μg
- 品牌 Brand
- Bioworld
Description
pa href=http://www.bioworlde.com/ACE2-%28K465%29-Antibodyimg alt= src=http://www.bioworlde.com/best/template/product/image/img11.gif //astock/p
pimg alt= src=file:///C:/Users/jimmy/AppData/Local/Temp/moz-screenshot-2.jpg //p
h3 class=list6_t1Host: Rabbit/h3
h3 class=list6_t1Reactivity: Human,Mouse,Rat/h3
p class=list6_t1nbsp;/p
div class=list6_li
h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
/div
div class=list6_li
h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
/div
div class=list6_li
h3 class=list6_t1Specificity/h3
p class=list6_textWNK1 (Y52) antibody detects endogenous levels of WNK1 protein./p
/div
div class=list6_li
h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: 1:500~1:1000 IHC: 1:50~1:200/p
/div
div id=pic
div id=westernpic
div class=imagecontainer
h3Western Blot(WB)/h3
img id=wpic src=http://www.bioworlde.com/upload/web/1374.jpg width=120 alt= /
div style=width: 250pxWestern blot (WB) analysis of WNK1 (Y52) antibody in extracts from 293 cells treated with EGF 200ng/ml 30'./div
/div
/div
div id=IHCpic
div class=imagecontainer
h3Immunohistochemistry (IHC)/h3
img id=ipic height=180 src=http://www.bioworlde.com/upload/ihc/1374.jpg width=240 alt= //div
pImmunohistochemistry (IHC) analyzes of WNK1 (Y52) antibody in paraffin-embedded human breast carcinoma tissue./p
/div
/div
div class=list6_li
h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4deg;C short term. Aliquot and store at -20deg;C long term. Avoid freeze-thaw cycles./p
/div
div class=list6_li
h3 class=list6_t1Background/h3
p class=list6_textThe protein kinase superfamily contains over a thousand proteins in 57 subfamilies that all share a catalytic core of 250ndash;300 amino acids organized in two domains. WNK kinases (With No lysine (K)) are serine-threonine protein kinases that contain a cysteine residue in place of a lysine residue in a family of proteins that traditionally contain a lysine following a short string of hydro-phobic residues. WNK kinases contain a lysine upstream of the traditional position, within a glycine string. This lysine functions as an anchor and orients ATP through interactions with the alpha and beta phosphoryl groups. The catalytic domains of WNK2, WNK3 and WNK4 are 95% homologous to WNK1. Human WNK1 maps to chromosome 12p13 and encodes a 2382 protein that is primarily expressed in heart, kidney, muscle and distal nephron. Human WNK3 maps to chromosome Xp11.21-p11.23 and encodes a protein that is primarily expressed in brain. Human WNK4 maps to chromosome 17q21-q22 and encodes a 1243 amino acid protein that is expressed in kidney./p
/div
pimg alt= src=file:///C:/Users/jimmy/AppData/Local/Temp/moz-screenshot-2.jpg //p
h3 class=list6_t1Host: Rabbit/h3
h3 class=list6_t1Reactivity: Human,Mouse,Rat/h3
p class=list6_t1nbsp;/p
div class=list6_li
h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
/div
div class=list6_li
h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
/div
div class=list6_li
h3 class=list6_t1Specificity/h3
p class=list6_textWNK1 (Y52) antibody detects endogenous levels of WNK1 protein./p
/div
div class=list6_li
h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: 1:500~1:1000 IHC: 1:50~1:200/p
/div
div id=pic
div id=westernpic
div class=imagecontainer
h3Western Blot(WB)/h3
img id=wpic src=http://www.bioworlde.com/upload/web/1374.jpg width=120 alt= /
div style=width: 250pxWestern blot (WB) analysis of WNK1 (Y52) antibody in extracts from 293 cells treated with EGF 200ng/ml 30'./div
/div
/div
div id=IHCpic
div class=imagecontainer
h3Immunohistochemistry (IHC)/h3
img id=ipic height=180 src=http://www.bioworlde.com/upload/ihc/1374.jpg width=240 alt= //div
pImmunohistochemistry (IHC) analyzes of WNK1 (Y52) antibody in paraffin-embedded human breast carcinoma tissue./p
/div
/div
div class=list6_li
h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4deg;C short term. Aliquot and store at -20deg;C long term. Avoid freeze-thaw cycles./p
/div
div class=list6_li
h3 class=list6_t1Background/h3
p class=list6_textThe protein kinase superfamily contains over a thousand proteins in 57 subfamilies that all share a catalytic core of 250ndash;300 amino acids organized in two domains. WNK kinases (With No lysine (K)) are serine-threonine protein kinases that contain a cysteine residue in place of a lysine residue in a family of proteins that traditionally contain a lysine following a short string of hydro-phobic residues. WNK kinases contain a lysine upstream of the traditional position, within a glycine string. This lysine functions as an anchor and orients ATP through interactions with the alpha and beta phosphoryl groups. The catalytic domains of WNK2, WNK3 and WNK4 are 95% homologous to WNK1. Human WNK1 maps to chromosome 12p13 and encodes a 2382 protein that is primarily expressed in heart, kidney, muscle and distal nephron. Human WNK3 maps to chromosome Xp11.21-p11.23 and encodes a protein that is primarily expressed in brain. Human WNK4 maps to chromosome 17q21-q22 and encodes a 1243 amino acid protein that is expressed in kidney./p
/div
同品牌其他產品
