w-Conotoxin MVIIC
- 貨號 Cat. No.
- C-150
- 規格 Size
- 30 μg 5x30 μg
Description
w-Conotoxin MVIIC
A Blocker of Presynaptic Cav (N-type and P/Q type) Channels
| Product#: | C-150 |
| Sizes: |
| 30 mg |
| 5 x 30 mg |
| M.W.: | 2756 |
| Sequence: | TRCKG KGAPC RKTMY DCCSG SCGRR GKCG |
| Purity: | >99% |
| Bioassay: |
w-Conotoxin MVIIC blocked cloned Cav2.2 channels transiently expressed in Xenopus oocytes.3 |
 |
Inhibition of Cav2.2 expressed in Xenopus oocytes by w-Conotoxin MVIIC. Left: Time course of current amplitude inhibition by application of 1 mM toxin (period of toxin perfusion is indicated by the bar). Right: Traces before (blue) and during (pink) application of the toxin. |
| References: |
1. | McDonough, S. I. et al. (1996) J. Neurosci. 16, 2612. |
2. | Liu, H. et al. (1996) J. Biol. Chem. 23, 13804. |
3. | Stocker, J. W. et al. (1997) J. Neurosci. 17, 3002. |
4. | Herrero, C. J. et al. (1999) Br. J. Pharmacol. 127, 1375. |
5. | Newcomb, R. and Palma, A. (1994) Brain. Res. 638,95. |
6. | Vega, T. et al. (1995) Eur. J. Pharmacol. 276,231. |
7. | Hirata, H. et al. (1997) Eur. J. Pharmacol. 321,217. |
w-Conotoxin MVIIC, blocks CaV2.1 (a1A, P/Q-type) and Cav2.2 (a1B, N-type) channels.1 The toxin binds with high affinity to Cav2.1 and with lower affinity to Cav2.2 in rabbit brain.2 However, the block by w-Conotoxin-MVIIC, of N-type channels in DRG neurons developed much faster then the block of P-type currents in Purkinje cells.1 The effect of the toxin is modulated by voltage (i.e. it is more potent for inactivated channels).3 In addition this toxin was reported to block nicotinic receptors (transiently expressed in Xenopus oocytes) with IC50 of 1.3 mM.4 It was also shown to inhibit K+-induced 3H-GABA release in hippocampus in vivo.5 This effect was with high affinity (50% block, 200 nM). The toxin was used to inhibit synaptic transmission in several peripheral preparations.6, 7