w-Conotoxin GVIA (Conus geographus)
- 貨號 Cat. No.
- C-300
- 規格 Size
- 0.1 mg 5x0.1mg 0.5mg 1mg
Description
w-Conotoxin GVIA (Conus geographus)
| Product#: | C-300 |
| Sizes: |
| 0.1 mg |
| 5 x 0.1 mg |
| 0.5 mg |
| 1 mg |
| M.W.: | 3037 |
| Sequence: | CKSXG SSCSX TSYNC CRSCN XYTKR CY (X = Hydroxyproline) |
| Purity: | >99% |
| Bioassay: |
 |
The biological activity of this toxin was tested to confirm its ability to block N-type CaV currents. 20 nM w-Conotoxin GVIA inhibited N-type CaV currents elicited in Xenopus oocytes injected with CaV2.2, a2d-1 and b2a mRNA. Left: Time course of toxin action. Current amplitudes are plotted as a function of the stimulus number. Oocytes membrane potential was held at ?00 mV and 100 ms stimulating pulse to 0 mV was delivered every 10 seconds. 20 nM toxin were perfused in the period marked by the bar. Right: Example traces of N-type currents before and during toxin application. |
| References: |
1. | Olivera, B.M. et al. (1984) Biochemistry 23 (22), 5087. |
2. | McCleskey, E. W. et al. (1987) Proc. Natl. Acad.Sci. USA 84, 4327. |
3. | Feng, Z. P. et al. (2001) J. Biol. Chem. 276, 15728. |
4. | Kerr, L. M. and Yoshikami, D. (1984) Nature 308, 282. |
5. | Lalo, U. V. et al. (2001) Brain Res. Bull. 54, 507. |
6. | Pravettoni, E. et al. (2000) Dev. Biol. 227, 581. |
w-Conotoxin GVIA is a synthetic toxin originally isolated from Conus geographus. w-Conotoxin GVIA is a specific blocker of Cav2.2 Ca2+ channels. It specifically blocks N-type Cav channels by binding to the Cav2.2 a1 subunit (a1B) and its action is only partially reversible.2,3 In accordance it inhibits synaptic transmission in many systems.4 It is also reported to antagonize P2X receptors.5 The toxin is used to specifically investigate Ca v2.2 channels contributions (by subtraction of the activity before and during perfusion of the toxin). Alternatively, it is used to eliminate the N-type channel contribution to highlight some other channel or enzyme activity. w-Conotoxin GVIA was used also to purify the channel protein using immunoprecipitation techniques and to label and localize channels and synapses (See for example).6