VTN Antibody (LS-B2118)

貨號 Cat. No.: LS-B2118
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2118
Antibody Validation:	This antibody replaces catalog number LS-C40239. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Vitronectin (VTN)
Synonyms:	VTN, vitronectin
Family:	Matrix Protein
Subfamily:	not assigned-Other
Host:	VTN antibody was produced in Mouse
Antigen Species:	VTN antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1,k
Antigen Type:	Purified protein
Immunogen:	VTN antibody was raised against human vitronectin purified from plasma by heparin-affinity chromatography.
Antibody Specificity:	Highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). Cross-reacts with vitronectin from cat, dog, goat, cow and to a lesser extent with horse. Epitope is located in the somatomedin B domain. Binds nearly as well to native vitronectin as to denatured. Is a potent inhibitor of integrin-mediated cell adhesion to vitronectin and a moderate inhibitor of PAI-1 binding. Also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well.
Predicted Species Reactivity:	Bovine, Cat, Dog, Goat, Horse
Purification:	Affinity Chromatography
Presentation:	PBS, pH 7.4, 500 mM sodium chloride, 15 mM sodium azide, 40% glycerol.
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2118 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2118 was determined to be 2.5 ug/ml. ELISA: 1:15000. Coat with vitronectin directly onto the microtiter well.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (2.5 µg/ml), Western blot (WB) (1:50), ELISA (1:30000) (Optimal dilution to be determined by the researcher)
Size:	50 µg