Vitamin D3 (human) ELISA kit
Kits
- 貨號 Cat. No.
- K4806-100
- 規格 Size
- K4806-100
- Reactivity
- Human
- Sample Type
- • Serum & plasma (EDTA/Citrate) • Urine • Cell culture medium 、 tissue homogenates and cell lysates
Description
Alternate Name : Human Vitamin D3 (Cholecalciferol) ELISA kit
Tag Line :
Sensitive, Colorimetric Assay
Storage Conditions: 4°C
Shelf life: 12 months
Summary :
• Detection method- Absorbance (450 nm)
• Species reactivity-human
• Application- Quantitative protein detection, establishing normal range.
Detection Method: Absorbance (450 nm)
Features & Benefits:
• Easy, convenient and time-saving method to assay for human vitamin D3.
• Detection Range:200 – 800 ug/L.
• This ELISA kit shows no species cross-reactivity.
Description:
Vitamin D3 (Cholecalciferol) is found in human epidermis and dermis. BioVision’s human Vit. D3 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human Vit. D3 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Vit. D3 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Vit. D3 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 20 – 800 µg/L.