Vimentin Antibody (LS-B1870)

Description

Antibody Catalog ID:	LS-B1870
Antibody Validation:	This antibody replaces catalog number LS-C46123. It has been validated for use in the following assays: IHC.
Antibody Target:	Vimentin
Synonyms:	VIM, vimentin
Host:	VIM antibody was produced in Mouse
Antigen Species:	VIM antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgM
Antibody Clone Name:	VI-10
Predicted Species Reactivity:	Chicken, Mouse, Porcine, Rat
Purification:	Size Exclusion Chromatography
Presentation:	PBS, 15 mM sodium azide, approx., pH 7.4.
Recommended Storage:	+4°C, avoid freezing
Antibody Usage Summary:	Immunohistochemistry: LS-B1870 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1870 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunocytochemistry (ICC), Western blot (WB), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	1 mg/ml