VEGF protein
- 貨號 Cat. No.
- VEGF protein
- 規格 Size
- 10ug
- 品牌 Brand
- gene-rl
Description
| Product Name | VEGF protein |
| Product type | Proteins and Peptides |
| Size | 10ug |
| Peptide | Recombinant full length protein, with a N-terminal hexahistidine tag. |
| Tested applications | SDS-PAGE, Western Blotting, Cell Test |
| Application notes | Recommended dilutions SDS-PAGE: Use at an assay dependent dilution Western Blotting: Use at an assay dependent dilution Optimal dilutions/concentrations should be determined by the end user. |
| Relevance | VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. |
| Storage buffer | n/a |
| Purity | >95% by SDS-PAGE and PLC analysis. |
| Form | Lyophilised |
| Storage instructions | Aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Expression system | Yeast (Eukaryotic cell) |
| Sequence | Human VEGF is a 38.2kDa protein consisting of two 165 amino acid residues: APMAEGGGQN HHEVVKFMDV YQRSYSHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR |
 |  |  |  | ||||||
0 ug/ml | 20 ng/ml | 200 ng/ml | 2 ug/ml | ||||||
| Fig 1: VEGF Treatment of HUVEC Cell Line 　　Different concentration of purified VEGF was added into HUVEC cell culture medium. 　　Pictures were taken after 24 or 48 hours. 　　The purified growth factor increased the proliferation of HUVEC cell line. ã€€ã€€ç”Ÿé•·å› å在HUVEC的細胞ä¸ç”¨ä¸åŒæ¿ƒåº¦åŸ¹é¤Š,æ–¼24或48å°æ™‚æ‹ç…§è§€å¯Ÿ ã€€ã€€ç”Ÿé•·å› åå¯å¢žåŠ HUVEC分化生長的能力 | |||||||||
| |||||||||