VEGF-D Antibody

Antibody Target: VEGF-D

Alternative Name: VEGFD, VEGF D, Vascular endothelial growth factor D, c-fos-induced growth factor, FIGF

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: E. coli expressed human VEGF-D

Accession#: D89630

Gene ID: 2277

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti- VEGF-D polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: human

Application: Western blot

Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human VEGF-D (Cat.# 4643-10) can be used as a positive control. Other applications have not been determined.

Storage Temp.: -20 °C

Shipping: gel pack

Shelf Life: 12 months

Background Descriptions: EGF-D is most closely related to VEGF-C by virtue of the presence of N- and C-terminal extensions that are not found in other VEGF family members. In adult human tissues, VEGF-D mRNA is most abundant in heart, lung, skeletal muscle, colon, and small intestine. Analyses of VEGF-D receptor specificity revealed that VEGF-D is a ligand for both VEGF receptors (VEGFRs) VEGFR-2 (Flk1) and VEGFR-3 (Flt4) and can activate these receptors. However, VEGF-D does not bind to VEGFR-1. Expression of a truncated derivative of VEGF-D demonstrated that the receptor-binding capacities reside in the portion of the molecule that is most closely related in primary structure to other VEGF family members and that corresponds to the mature form of VEGF-C. In addition, VEGF-D is a mitogen for endothelial cells. The recombinant VEGF-D as the immunogen was expressed based on sequence from Genebank Accession# D89630, 20-354 aa.

Handling: The antibody solution should be gently mixed before use.