進階篩選 ›

Vector-Based miRNA Services 載體為基礎的miRNA的服務

貨號 Cat. No.
SC1080
規格 Size
客製化

Description

microRNA （微RNA ）小於（ 〜 22個核苷酸）非編碼RNA的表達metazoans和病毒。他們的目標mRNA的調節posttranscriptionally指示卵裂和退化後者如 果有充分的互補性，或通過抑制翻譯起始的互補性，如果是不完善的。微RNA是從初級加工謄稱為初級miRNA的，短莖環結構稱為前的miRNA ，並最終功能的miRNA 。

miRNA的服務



一些獨特的物理屬性的微RNA ，如規模小，缺乏聚adenylated尾巴，使它們難以實現和具有挑戰性的研究。經過多年的經驗和專業知識， GenScript開發了一個先進的基於載體的miRNA的服務，可以幫助緩解困難。由此產生的miRNA表達質粒純化，並準備轉，可用於多種應用，以降低調節基因表達。


miRNA service

主要特點：



     *全面的miRNA載體收集

     *自定義成本效益的再生載體

     *強沉默效應

     *快速，可靠的結果具有競爭力的價格



















Service Type Service Description Delivery Details

Vector based pri-miRNA construction

  1. Our optimized SV40 -CMV promoter causes increased expression of pre-miRNA

  2. Selection of cGFP fluorescent tags

  3. Final product is confirmed by stringent sequencing

  1. 4 μg of lyophilized plasmid DNA containing the desired miRNA construct

  2. Detailed sequencing data documenting the DNA sequence of each clone

  3. The typical delivery time is about two weeks.

Vector-based pre-miRNA construction

  1. Selection of cGFP fluorescent tags

  2. Final product is confirmed by stringent sequencing



注： GenScript的載體是完全兼容的腺病毒和慢病毒載體系統。


Gene Synthesis (* Required fields) send

Gene Information:


* Gene name:
















5' sequence:
* Sequence: DNA   Protein Length   
3' sequence:


 




 


Codon Optimization:
















  Do you need codon optimization (Complimentary)?
 Yes
 No  



Cloning Strategy:















pUC57 Cloning site:    pUC57 vector map
   (Example: BamHI-HindIII, EcoR V; Note: If leave it blank, an available site will be chosen to insert the gene by GenScript, usually EcoR V)
Other

 








Other Requirements:



 








Plasmid Preparation:






















Standard delivery: 4 ug (Free of charge)
Custom plasmid preparation:
100 ug ( $50 )
200 ug
500 ug
1 mg
2 mg
10 mg
20 mg
50 mg
100 mg
200 mg
500 mg
1000 mg
1500 mg
2000 mg
Other
  Endotoxin grade:
Molecular
Low Endotoxin (<0.03 EU/ug)
Ultralow Endotoxin (<0.005 EU/ug)
  Additional analysis:

Bio-Burden Assay
Endotoxin Assay (LAL test)
Restriction Analysis



Additional Services:


  GenScript provides a series of additional premium services at very competitive prices.


 


  Please choose one or more options of the following:


 












Plasmid preparation
Mutagenesis

詢價 / 洽詢此產品

回到產品列表

▲ LINE
諮詢