Proteins and Peptides
VDR Antibody (LS-B2577)
- 貨號 Cat. No.
- LS-B2577
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2577 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C11024. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | VDR |
| Synonyms: | VDR Antibody, 1,25-dihydroxyvitamin d3 receptor Antibody, nr1i1 Antibody, vdr Antibody, Vitamin D Receptor Antibody, Vitamin d3 receptor Antibody |
| Family: | NHR |
| Subfamily: | NR1 Thyroid hormone-like |
| Host: | VDR antibody was produced in Rat |
| Antigen Species: | VDR antibody was raised against Chicken |
| Clonality: | Monoclonal |
| Isotype: | IgG2b |
| Antigen Type: | Purified protein |
| Immunogen: | VDR antibody was raised against vitamin D receptor protein from chicken intestine (53kD). Cellular Localization: Nuclear. Epitope: 89-105. |
| Antibody Specificity: | Recognizes both the occupied and unoccupied forms of VDR. Does not cross-react with estrogen and glucocorticoid receptors. In mammals, recognizes a protein of ~53kD which is identified as vitamin D Receptor (VDR). In avians, VDR appears as a doublet of 58 and 60kD. Species cross-reactivity: Human, porcine, mouse, rat, hamster, frog, chicken and fish. |
| Antigen Modification: | aa89-105 |
| Predicted Species Reactivity: | Chicken, Frog, Hamster, Human, Mouse, Porcine, Rat |
| Purification: | Protein G Column |
| Presentation: | PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2577 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2577 was determined to be 10 ug/ml. Positive control: LNCaP and HeLa cells. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (1 - 2 µg/ml), Gel shift (GS) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 0.2 mg/ml |
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