VCL ELISA Kit (Human)

pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Gene Symbol:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;a href=http://www.ncbi.nlm.nih.gov/gene/7414 target=_blank style=margin: 0px; padding: 0px; color: rgb(6, 53, 122); font-weight: bold;VCL/a/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Official Gene Full Name:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;vinculin/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;NCBI Gene Id:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;7414/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Alias Symbols:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;CMD1W, CMH15, Metavinculin, MVCL, Vinculin/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Description Of Target:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.nbsp;/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Protein Name:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;Vinculin/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Swissprot Id:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;a href=http://www.uniprot.org/uniprot/P18206 target=_blank style=margin: 0px; padding: 0px; color: rgb(6, 53, 122); font-weight: bold;P18206/a/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Protein Accession #:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;a href=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=proteinamp;cmd=searchamp;term=NP_003364.1 target=_blank style=margin: 0px; padding: 0px; color: rgb(6, 53, 122); font-weight: bold;NP_003364.1/a/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Nucleotide Accession #:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;a href=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Nucleotideamp;cmd=searchamp;term=NM_003373.3 target=_blank style=margin: 0px; padding: 0px; color: rgb(6, 53, 122); font-weight: bold;NM_003373.3/a/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Species Reactivity:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;Human/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Sample Type:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Sensitivity:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;lt; 0.78 ng/mL/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Kit Range:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;1.56 - 100 ng/mL/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Kit Reproducibility:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;Mean Intra-assay CV%: lt; 4.6% (n = 20)br style=margin: 0px; padding: 0px; /
Mean Inter-assay CV%: lt; 7.6% (n = 20)/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Kit Duration:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;~ 3 Hours/dd
/div
pnbsp;/p
div class=dl_cover style=margin: 0px; padding: 0px 0px 5px; width: 680px; display: inline-block; position: relative; color: rgb(104, 104, 104); font-family: Arial, sans-serif, Helvetica; background-color: rgb(251, 250, 246);
dt style=margin: 0px; padding: 0px; text-transform: capitalize; width: 272px; float: left; clear: both; font-size: 13px;Kit Principle:/dt
dd style=margin: 0px 0px 9px 13.5938px; padding: 0px; word-wrap: break-word; width: 394.391px; white-space: pre-wrap; float: left; position: relative;Aviva Systems Biology VCL ELISA Kit (Human) (OKEH01312) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VCL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for VCL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VCL captured in well./dd
/div