VASP Antibody

Antibody Target: VASP

Alternative Name: Vasodilator-stimulated phosphoprotein

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Synthetic peptide surrounding amino acid 371 of human VASP

Accession#: P50552

Gene ID: 7408

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in 1X phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: human, mouse, rat

Application: Western blot

Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody recognizes ~46 kDa VASP from samples of human, mouse, and rat origins. Reactivity to other species has not been tested.

Storage Temp.: -20 °C

Shipping: gel pack

Shelf Life: 12 months

Background Descriptions: Vasodilator-stimulated phosphoprotein (VASP) belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways. VASP functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance. Three phosphorylation sites, Ser157, Ser239 and Thr278, have been identified. Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization.

Handling: The antibody solution should be gently mixed before use.