V70A Ubiquitin
- 貨號 Cat. No.
- UM-V70A
- 規格 Size
- 1 mg
- 品牌 Brand
- Boston Biochem
Description
V70A Ubiquitin
human recombinant
Product Information
1 mg
Soluble and stable in aqueous buffers up to 10 mg/ml.
8.5 kDa
> 95% by SDS-PAGE
This is a lyophilized powder
Use & Storage
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.
Catalog #: UM-V70A
V70A Ubiquitin
Product Source:
human recombinant
Ubiquitin has a distinct and functionally important hydrophobic patch whose surface is defined by three residues including Leu8, Ile44 and Val70. These residues are solvent accessible in ubiquitin chains and are critical for poly-ubiquitin chain interaction and recognition by the 26S proteasome and subsequent target degradation. These hydrophobic residues interact directly with various factors that bind to Ub and/or mediate Ub-Ub interactions in ubiquitin chains that may influence orientation and recognition. Ubiquitin V70A can form an E1-catalyzed active thioester at the
C-terminus allowing the molecule to be transferred to the lysines of substrate proteins.
Product Information
Quantity:
1 mg
Stock:
Soluble and stable in aqueous buffers up to 10 mg/ml.
MW:
8.5 kDa
Purity:
> 95% by SDS-PAGE
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
Storage:
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.